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Cryo-EM Motion Corrected Micrographs of Human Prothrombin:Prothrombinase

ABSTRACT:

SUBMITTER: Enrico Di Cera

PROVIDER: EMPIAR-11615 | biostudies-other |

REPOSITORIES: biostudies-other

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Cryo-EM structure of the prothrombin-prothrombinase complex.

Ruben Eliza A EA Summers Brock B Rau Michael J MJ Fitzpatrick James A J JAJ Di Cera Enrico E

Blood 20220601 24

The intrinsic and extrinsic pathways of the coagulation cascade converge to a common step where the prothrombinase complex, comprising the enzyme factor Xa (fXa), the cofactor fVa, Ca2+ and phospholipids, activates the zymogen prothrombin to the protease thrombin. The reaction entails cleavage at 2 sites, R271 and R320, generating the intermediates prethrombin 2 and meizothrombin, respectively. The molecular basis of these interactions that are central to hemostasis remains elusive. We solved 2 ...[more]

PMID: 35427420

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