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Cryo-EM structure of Pseudomonas Aeruginosa TonB-dependent transporter PhuR in complex with synthetic antibody and heme

ABSTRACT:

SUBMITTER: Satchal Krishna Erramilli

PROVIDER: EMPIAR-11627 | biostudies-other |

REPOSITORIES: biostudies-other

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Chaperone-assisted cryo-EM structure of P. aeruginosa PhuR reveals molecular basis for heme binding.

Knejski Paweł P PP Erramilli Satchal K SK Kossiakoff Anthony A AA

Structure (London, England : 1993) 20240206 4

Pathogenic bacteria, such as Pseudomonas aeruginosa, depend on scavenging heme for the acquisition of iron, an essential nutrient. The TonB-dependent transporter (TBDT) PhuR is the major heme uptake protein in P. aeruginosa clinical isolates. However, a comprehensive understanding of heme recognition and TBDT transport mechanisms, especially PhuR, remains limited. In this study, we employed single-particle cryogenic electron microscopy (cryo-EM) and a phage display-generated synthetic antibody ( ...[more]

PMID: 38325368

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