Cryo-EM structure of T. pseudonana PyShell helical tube
Ontology highlight
ABSTRACT:
SUBMITTER: Akihiro Kawamoto
PROVIDER: EMPIAR-11724 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Diatom pyrenoids are encased in a protein shell that enables efficient CO<sub>2</sub> fixation.
Shimakawa Ginga G Demulder Manon M Flori Serena S Kawamoto Akihiro A Tsuji Yoshinori Y Nawaly Hermanus H Tanaka Atsuko A Tohda Rei R Ota Tadayoshi T Matsui Hiroaki H Morishima Natsumi N Okubo Ryosuke R Wietrzynski Wojciech W Lamm Lorenz L Righetto Ricardo D RD Uwizeye Clarisse C Gallet Benoit B Jouneau Pierre-Henri PH Gerle Christoph C Kurisu Genji G Finazzi Giovanni G Engel Benjamin D BD Matsuda Yusuke Y
Cell 20241001 21
Pyrenoids are subcompartments of algal chloroplasts that increase the efficiency of Rubisco-driven CO<sub>2</sub> fixation. Diatoms fix up to 20% of global CO<sub>2</sub>, but their pyrenoids remain poorly characterized. Here, we used in vivo photo-crosslinking to identify pyrenoid shell (PyShell) proteins, which we localized to the pyrenoid periphery of model pennate and centric diatoms, Phaeodactylum tricornutum and Thalassiosira pseudonana. In situ cryo-electron tomography revealed that pyren ...[more]
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