Dataset Information
SpCas9 bound to 18 nucleotide complementary DNA substrate in the catalytic state
Ontology highlight
ABSTRACT:
SUBMITTER: Martin Jinek
PROVIDER: EMPIAR-11783 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
R-loop formation and conformational activation mechanisms of Cas9.
Nature 20220824 7925
Cas9 is a CRISPR-associated endonuclease capable of RNA-guided, site-specific DNA cleavage<sup>1-3</sup>. The programmable activity of Cas9 has been widely utilized for genome editing applications<sup>4-6</sup>, yet its precise mechanisms of target DNA binding and off-target discrimination remain incompletely understood. Here we report a series of cryo-electron microscopy structures of Streptococcus pyogenes Cas9 capturing the directional process of target DNA hybridization. In the early phase o ...[more]