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Plant SBF-SEM - Tobacco Leaf Chloroplast


ABSTRACT:

SUBMITTER: Kirk James Czymmek 

PROVIDER: EMPIAR-11831 | biostudies-other |

REPOSITORIES: biostudies-other

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Publications

A conventional fixation volume electron microscopy protocol for plants.

Wickramanayake Janithri S JS   Czymmek Kirk J KJ  

Methods in cell biology 20230614


Volume electron microscopy techniques play an important role in plant research from understanding organelles and unicellular forms to developmental studies, environmental effects and microbial interactions with large plant structures, to name a few. Due to large air voids central vacuole, cell wall and waxy cuticle, many plant tissues pose challenges when trying to achieve high quality morphology, metal staining and adequate conductivity for high-resolution volume EM studies. Here, we applied a  ...[more]

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