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Exploring the 3D structure of chromosomes in mitotic cells


ABSTRACT:

SUBMITTER: William C Earnshaw 

PROVIDER: EMPIAR-11953 | biostudies-other |

REPOSITORIES: biostudies-other

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Near millimolar concentration of nucleosomes in mitotic chromosomes from late prometaphase into anaphase.

Cisneros-Soberanis Fernanda F   Simpson Eva L EL   Beckett Alison J AJ   Pucekova Nina N   Corless Samuel S   Kochanova Natalia Y NY   Prior Ian A IA   Booth Daniel G DG   Earnshaw William C WC  

The Journal of cell biology 20240826 11


Chromosome compaction is a key feature of mitosis and critical for accurate chromosome segregation. However, a precise quantitative analysis of chromosome geometry during mitotic progression is lacking. Here, we use volume electron microscopy to map, with nanometer precision, chromosomes from prometaphase through telophase in human RPE1 cells. During prometaphase, chromosomes acquire a smoother surface, their arms shorten, and the primary centromeric constriction is formed. The chromatin is prog  ...[more]

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2016-09-27 | PXD003877 | Pride