Diverse intratumoral heterogeneity and immune microenvironment of two HPV-related cervical cancer types revealed by single cell transcriptomic profiling

ABSTRACT: Cervical squamous cell carcinoma (SCC) and adenocarcinoma (AD) are the main histological types of HPV-related cervical cancer (CC). However, reports on cell type-specific molecular difference between SCC and AD are scanty. Here, we use unbiased droplet-based single-cell RNA sequencing (RNA-seq) to elucidate the cellular differences between SCC and AD in tumor heterogeneity, and tumor microenvironment (TME). A total of 61,723 cells from three SCC and three AD patients, were collected and classified into nine cell types. The tumor cells between SCC and AD showed high intra- and inter-patient heterogeneity and functional diversity. Signaling pathways, such as epithelial-to-mesenchymal-transition (EMT), hypoxia and inflammatory response were up-regulated in SCC, while cell cycle-related signal

ORGANISM(S): Homo sapiens (human)

SUBMITTER:  

PROVIDER: S-BSST1035 | biostudies-other |

REPOSITORIES: biostudies-other