The role of gut microbiota in mediating tissue injury through the promotion of proteolysis and heme bioavailability in the small intestine

ABSTRACT: Background: Excessive meat intake is considered to be associated with multiple intestinal injuries. However, the specific mechanism is still controversial. Results: It was found that pork diet (typical meat diet) only induced small intestinal injury in specific-pathogen free (SPF) mice but not in germ-free (GF) mice, highlighting the indispensable role of gut microbiota in intestinal health. ELISA and RT-qPCR indicated that the intestinal tissue in GF mice had lower levels of heme and Hmox1 compared with the SPF mice, suggesting the low bioavailability of myoglobin without symbiotic microbes. High throughput sequencing showed that dietary myoglobin and heme effected intestinal flora diversity, with Lactobacillus, Muribaculaceae and Bifidobacterium being enriched by myoglobin. Additionally,

ORGANISM(S): Mus musculus (mouse)

SUBMITTER:  

PROVIDER: S-BSST1056 | biostudies-other |

SECONDARY ACCESSION(S): PRJNA948118

REPOSITORIES: biostudies-other