Transcriptomic effect of Anethole trithione (ATT) on human BPH-1 cells

ABSTRACT: The goal of this experiment was to evaluate the effect of antioxidant treatment on the transcriptome of epithelial cells from human benign prostate hyperplasia (BPH). The BPH-1 cell line has been immortalized from a 68-year-old BPH patient using SV-40 T-antigen (Hayward et al 1995, In Vitro Cell Dev Biol Anim 31, 14-24). BPH-1 cells were purchased in 2019 at DSMZ-German collection (ACC 143, reference 1402) and maintained in DMEM medium (Gibco, GlutamaxTM, 4.5g/L D-Glucose + Pyruvate, reference 31966-0.21) with 10% FBS (Eurobio, CVFSVF00-01). Anethole trithione (ATT) is an antioxidant molecule acting as a specific inhibitor of reactive oxygen species (ROS) production at the Iq site of the mitochondrial respiratory chain (Detaille et al, PLoS One 14, e0216385). ATT was initially resuspende

ORGANISM(S): Homo sapiens (human)

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PROVIDER: S-BSST1129 | biostudies-other |

REPOSITORIES: biostudies-other