Validation of protein-RNA interaction using the protein microarray

ABSTRACT: The HuProt Human proteome microarray contains over 21,000 GST-purified unique recombinant human proteins in yeast, including >81% of the canonically expressed proteins as defined by the Human Protein Atlas. Tests on the HuProt protein microarray have showed that proteins are folded in their native conformation and retain function, such as protein-RNA binding, protein-DNA binding, etc. Therefore, the HuProt protein microarray can be an alternative tool to study the RNA-binding activities of proteins. We generated a pool of Cy5-labelled RNAs by mixing the fragmented total RNAs (13.5%) and in vitro transcribed RNAs (86.5%). To amplify the non-ribosomal RNAs (non-rRNAs), we generated the cDNA by reverse transcription using the total RNAs of HEK293 cells from which the ribosomal RNAs were fir

ORGANISM(S): Homo sapiens (human)

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PROVIDER: S-BSST1172 | biostudies-other |

REPOSITORIES: biostudies-other