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Analysis of the pathogen load and resistome of hospital surface microbiota

ABSTRACT: The persistent contamination of hospital surfaces contributes to the transmission of the so-called Healthcare-Associated Infections (HAI). We analysed the modulations induced by a sanitation system on the presence of six pathogen groups on the surfaces of six Italian hospitals (Staphylococci, Enterobacteriaceae, Mycetes, Klebsiella, Acinetobacter, Psudomonas). The analyses were performed by direct CFU count on Rodac plates used for surface sampling. Monthly samplings were performed in 3-6 rooms per hospital ward, in 3 different points (floor, bed and sink). After incubationfor 24-72 h, CFU were counted and values expressed as CFU/m2. In parallel, the antibiotic-resistance genes of the whole population contaminating hospital surfaces were analysed and quantified by a commercial microarray using real-time quantitative PCR and Taqman probe for 84 different resistance genes (Qiagen, Antibiotic Resistance Genes, BAID-1901ZRA, Hilden, Germany). The study is part of a 18-months trial performed to evidence the impact of a sanitation system on the modulation of surface microbiota and the consequent impact on healthcare-associated infection transmission (Trial registration - ISRCTN International Clinical Trials Registry, ISRCTN58986947).

SUBMITTER: Elisabetta Caselli

PROVIDER: S-BSST75 | biostudies-other |

REPOSITORIES: biostudies-other

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