18S rDNA miTags information for Tara Oceans Expedition (2009-2013), including the Tara Polar Circle Expedition (2013)

ABSTRACT: 18S rRNA gene miTags (metagenomic Illumina tags, i.e., 18S rRNA gene assemblies derived from metagenomes sequenced with an Illumina platform) for sizes fractions >0.8 µm (0.8-5 µm, 0.8-2000 µm, 3-20 µm, 5-20 µm, 20-180 µm, 180-2000 µm). We extracted metagenomic reads for rRNA using SortMeRNA (Kopylova, Noé & Touzet 2012 Bioinformatics 28.24: 3211-3217) and those with ≥100 bp length with no Ns were dereplicated at the study level using VSEARCH v2.18 (Rognes et al. 2016 PeerJ 4:e2584). The resulting unique sequences were pairwise compared to PR2 v4.14 (Guillou at al. 2013 41.D1: D597-D604) using the VSEARCH's command --usearch_global to find the best hit defined as the reference sequence with the least differences in the region covering 100 percent of the query sequence. Only hits with ≥80%

ORGANISM(S): eukaryotes protists plankton

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PROVIDER: S-BSST762 | biostudies-other |

SECONDARY ACCESSION(S): PRJEB402

REPOSITORIES: biostudies-other