Lipid mediator profiling in inflammatory peritoneal exudates in sham vs. vagus nerve stimulated mice

ABSTRACT: Male (age 10-16 weeks) C57BL/6 (wild-type) (Charles River Laboratories), α7 nicotinic receptor deficient (C57BL/6 background, B6.129S7-Chrna7tm1Bay/J, Stock No. 003232 from The Jackson Laboratory) and Alox15-deficient (C57BL/6 background, B6.129S2-Alox15tm1Fun/J, Stock No. 002778 from The Jackson Laboratory) mice were used. After 1 hour of recovery from vagus nerve stimulation or sham surgery, 0.1 mg zymosan (Sigma-Aldrich, MO, USA) was administered intraperitoneally. Mice were euthanized by carbon dioxide asphyxiation at 4, 12, 24, and 48 hours after zymosan challenge. Peritoneal exudates were collected by injecting 4 mL of PBS intraperitoneally, shaking the abdomen, and recovering approximately 3 mL of exudate. Samples were extracted and lipid mediators were identified and quantified. I

ORGANISM(S): Mus musculus (mouse) Rattus norvegicus (brown rat)

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PROVIDER: S-BSST837 | biostudies-other |

REPOSITORIES: biostudies-other