Differentiation of main duct-IPMN from chronic pancreatitis by EUS and duct fluid cfDNA Next Generation Sequencing: a feasibility study

ABSTRACT: Background: A dilated main pancreatic duct (MPD) ≥ 5 mm can be observed in main duct (MD)-Intraductal Papillary Mucinous Neoplasm (IPMN) and chronic pancreatitis (CP). Distinction of both conditions might be difficult if imaging alone is used as a diagnostic tool. Cell-free DNA in MPD fluid obtained by Endoscopic Ultrasound (EUS)-guided fine needle aspiration (FNA) might help to distinguish MD-IPMN from CP. Methods: All patients with a dilated MPD ≥ 5 mm in EUS were prospectively analysed in this single centre study. EUS-guided pancreatic duct fluid aspiration was performed when MD-IPMN or CP was suspected in patients fit for surgery. Fourteen hot spot regions of known oncogenes or tumor suppressor genes (AKT1, BRAF, CTNNB1, EGFR, ERBB2, FBXW7, GNAS, KRAS, MAP2K1, NRAS, PIK3CA, SMAD4, TP

ORGANISM(S): Homo sapiens (human)

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PROVIDER: S-BSST965 | biostudies-other |

REPOSITORIES: biostudies-other