Transcription profiling of human fibrotic liver samples

ABSTRACT: Human liver samples were obtained from Analytical Biological Services Inc.(Wilmington, DE, USA) via autopsy 4-10 hours post-mortem with appropriate consent. The specimens were quickly frozen and stored at -80C. Following pulverization of the liver specimens total RNA was isolated by QIAzol extraction and RNeasy Mini Kit(QIAGEN, Valencia, CA, USA) purification. The RNA was then amplified via Ambion
5X MessageAmp II aRNA Amplification Kit (Austin, TX, USA) and profiled using Agilent Human Whole Genome Microarray Kit (Agilent Technologies, Santa Clara, CA, USA).

ORGANISM(S): Homo sapiens

SUBMITTER:  

PROVIDER: S-DIXA-D-1181 | biostudies-other |

REPOSITORIES: biostudies-other