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Transcription profiling by array of human melanoma cells with doxycycline-inducible RNAi-mediated knockdown of B-Raf

ABSTRACT: Unsupervised hierarchical clustering revealed a strong similarity in gene modulation resulting from either compound treatment or BRAF ablation mediated by RNA interference relative to DMSO-treated control samples . Experiment Overall Design: We have generated melanoma A375 cells stably expressing a shRNA construct for doxycycline-inducible knockdown of B-Raf (2 mg/ml Dox, 48h). Small molecule treatment was at 1000nM R341787, 24h.

ORGANISM(S): Homo sapiens

SUBMITTER: Hoeflich KP

PROVIDER: S-ECPF-GEOD-13487 | biostudies-other | 2009 Apr

REPOSITORIES: biostudies-other

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Transcription profiling by array of human melanoma cell lines

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