Anti-proliferative tumor suppressor-like activity of mir-9 overexpression and identification of novel mir-9 targets by trancription profiling in breast cancer cells
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Selcuklu SD
PROVIDER: S-ECPF-GEOD-33952 | biostudies-other | 2012 Aug
REPOSITORIES: biostudies-other
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Project description:Investigation of genome-wide effects (transcriptome profiling) and direct targets of mir-9 in MCF-7 cells transfected with mir-9 in two dosages (1X and 2X)
2012-09-04 | GSE33952 | GEO
Project description:Investigation of genome-wide effects (transcriptome profiling) and direct targets of mir-9 in MCF-7 cells transfected with mir-9 in two dosages (1X and 2X) Following transfections of mir-9 or control miRNA mimics, total RNA was isolated and microarray profiling was performed.
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Project description:MicroRNAs (miRNAs) play important roles in diverse biological processes and are emerging as key regulators of tumorigenesis and tumor progression. To explore the dysregulation of miRNAs in breast cancer, a genome-wide expression profiling of 939 miRNAs was performed in 50 breast cancer patients. A total of 35 miRNAs were aberrantly expressed between breast cancer tissue and adjacent normal breast tissue and several novel miRNAs were identified as potential oncogenes or tumor suppressor miRNAs in breast tumorigenesis. miR-125b exhibited the largest decrease in expression. Enforced miR-125b expression in mammary cells decreased cell proliferation by inducing G2/M cell cycle arrest and reduced anchorage-independent cell growth of cells of mammary origin. miR-125b was found to perform its tumor suppressor function via the direct targeting of the 3'-UTRs of ENPEP, CK2-?, CCNJ, and MEGF9 mRNAs. Silencing these miR-125b targets mimicked the biological effects of miR-125b overexpression, confirming that they are modulated by miR-125b. Analysis of ENPEP, CK2-?, CCNJ, and MEGF9 protein expression in breast cancer patients revealed that they were overexpressed in 56%, 40-56%, 20%, and 32% of the tumors, respectively. The expression of ENPEP and CK2-? was inversely correlated with miR-125b expression in breast tumors, indicating the relevance of these potential oncogenic proteins in breast cancer patients. Our results support a prognostic role for CK2-?, whose expression may help clinicians predict breast tumor aggressiveness. In particular, our results show that restoration of miR-125b expression or knockdown of ENPEP, CK2-?, CCNJ, or MEGF9 may provide novel approaches for the treatment of breast cancer.
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