Expression data from MCF7 and LTED cells treated with YC-1
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ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Aguilar Helena
PROVIDER: S-ECPF-GEOD-38829 | biostudies-other |
REPOSITORIES: biostudies-other
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Project description:To identify novel therapeutic opportunities for patients with acquired resistance to endocrine treatments in breast cancer, we applied a high-throughput drug screen. The IC50 values were determined for MCF7 and MCF7-LTED cells.
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Project description:To identify novel therapeutic opportunities for patients with acquired resistance to endocrine treatments in breast cancer, we applied high-throughput screening to explore currently marketed drugs. The Ec50 values were determined for MCF7 and LTED cell lines to identify the compounds showing higher inhibition of LTED cells. The best compound was YC-1 and gene microarray studies were done in vitro for mechanistic exploration. MCF7 and LTED cells were treated with YC-1 for RNA extraction and hybridization on Affymetrix microarrays.
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