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MiRNA profiling of GFAP+ cells isolated from GBM and adjacent non-tumor glial brain tissues

ABSTRACT: To identify miRNAs expression changes in human gliomas, we conducted a comprehensive microarray analysis to compare miRNA expression profiles in primary cells obtained from matched pairs of GBM and adjacent non-tumor brain tissues from 6 patients. Paired primary glioma cells and astrocytes, isolated from GBM tissues andperipheral non-tumor glial brain tissues, respectively, with each pair prepared from a same patient, were established for primary culture. Total RNA was extracted and hybridized to microarrays overnight at 65°C.

ORGANISM(S): Homo sapiens

SUBMITTER: Wang Huibo

PROVIDER: S-ECPF-GEOD-44726 | biostudies-other |

REPOSITORIES: biostudies-other

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miRNA profiling of GFAP+ cells isolated from GBM and adjacent non-tumor glial brain tissues

Project description:To identify miRNAs expression changes in human gliomas, we conducted a comprehensive microarray analysis to compare miRNA expression profiles in primary cells obtained from matched pairs of GBM and adjacent non-tumor brain tissues from 6 patients.

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miRNA profiling of GFAP+ cells isolated from GBM and adjacent non-tumor glial brain tissues

Project description:To identify miRNAs expression changes in human gliomas, weM-bM-^@M-^Bconducted a comprehensive microarray analysis to compare miRNAM-bM-^@M-^BexpressionM-bM-^@M-^BprofilesM-bM-^@M-^BinM-bM-^@M-^Bprimary cellsM-bM-^@M-^BobtainedM-bM-^@M-^BfromM-bM-^@M-^BmatchedM-bM-^@M-^BpairsM-bM-^@M-^BofM-bM-^@M-^BGBMM-bM-^@M-^BandM-bM-^@M-^BadjacentM-bM-^@M-^Bnon-tumorM-bM-^@M-^BbrainM-bM-^@M-^BtissuesM-bM-^@M-^BfromM-bM-^@M-^B6M-bM-^@M-^Bpatients. PairedM-bM-^@M-^Bprimary glioma cells andM-bM-^@M-^Bastrocytes, isolated from GBM tissues andperipheral non-tumor glial brain tissues, respectively, with each pair prepared from aM-bM-^@M-^Bsame patient,M-bM-^@M-^Bwere established for primary culture. Total RNA was extracted and hybridized to microarrays overnight at 65M-BM-0C.

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