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Genome-wide methylation and expression analysis of two breast cancer cell lines [RNA-Seq]

ABSTRACT: To improve our understanding of the relationships between methylation and expression we profiled mRNA expression and single-base resolution methylation levels for two breast cancer cell lines, MCF7 and T47D. Expression was profiled using RNA-seq. Methylation was assayed using Methyl-MAPS, which uses methylation-sensitive and -dependent restriction enzyme digests followed by high-throughput sequencing to identify methylation levels at individual CpGs (Edwards et al. 2010, Genome Research). RNA-Seq was used to generate mRNA expression profiles of MCF7 and T47D cells under standard growth conditions.

ORGANISM(S): Homo sapiens

SUBMITTER: Vanderkraats ND

PROVIDER: S-ECPF-GEOD-45335 | biostudies-other | 2013 Aug

REPOSITORIES: biostudies-other

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Genome-wide methylation and expression analysis of two breast cancer cell lines

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Genome-wide methylation and expression analysis of two breast cancer cell lines

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