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Expression data from H1299 human non-small cell lung carcinoma cell lines stably expressing CHES1 compared with H1299 infected with an empty vector

ABSTRACT: The expression of the forkhead transcription factor CHES1, also known as FOXN3, is reduced in many types of cancers. In vitro, CHES1 expression suppresses cell proliferation in tumor cell lines but not in normal cells. Conversely shRNA-mediated depletion of CHES1 increases tumor cell proliferation. We used microarrays to characterize the gene expression changes induced by CHES1 in H1299 cancer cells H1299 cells were infected with a retroviral vector that expresses CHES1 or an empty vector. After 5 days of infection, RNA was extracted from three independent replicas of each condition. Total RNA was send to Genome Quebec service for hybridization with Affymetrix microarrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Vernier Mathieu

PROVIDER: S-ECPF-GEOD-49422 | biostudies-other |

REPOSITORIES: biostudies-other

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Expression data from H1299 human non-small cell lung carcinoma cell lines stably expressing CHES1 compared with H1299 infected with an empty vector

2013-08-01 | GSE49422 | GEO

Expression data from H1299 human non-small cell lung carcinoma cell lines stably expressing CHES1 compared with H1299 infected with an empty vector

Project description:The expression of the forkhead transcription factor CHES1, also known as FOXN3, is reduced in many types of cancers. In vitro, CHES1 expression suppresses cell proliferation in tumor cell lines but not in normal cells. Conversely shRNA-mediated depletion of CHES1 increases tumor cell proliferation. We used microarrays to characterize the gene expression changes induced by CHES1 in H1299 cancer cells H1299 cells were infected with a retroviral vector that expresses CHES1 or an empty vector. After 5 days of infection, RNA was extracted from three independent replicas of each condition. Total RNA was send to Genome Quebec service for hybridization with Affymetrix microarrays.

2013-08-01 | E-GEOD-49422 | biostudies-arrayexpress

Expression data of NCI-H441 cells stably expressing hsa-mir-365-2 vs empty vector

Project description:Hsa-mir-365-2 is one of the two precursors that give rise to miR-365. We discovered that miR-365 directly regulates a lung cancer and developmental gene termed thyroid transcription factor 1 (TTF-1 or NKX2-1). Hsa-mir-365-2 was transfected into NCI-H441 cells via retrovirus-mediated gene transfer. Arrays hybridized: Affymetrix GeneChip Human Gene 1.0 ST Array. A total of 4 samples were analyzed. The first two were H441 cells expressing hsa-mir-365-2 and the other two were H441 transfected with empty vector. The class comparison function (significant cutoff, 0.001) of BRB Array Tool (4.1.0 beta_3 release) was used to analyze for differentially expressed genes as a result of enforced expression of hsa-mir-365-2.

| S-ECPF-GEOD-33672 | biostudies-other

Establishment of rat bone mesenchymal stem cell lines stably expressing Chondromodulin I.

Project description:To study the role of Chondromodulin I (ChM-I) in inducing bone mesenchymal stem cells (MSCs) into chondrocytes, the plasmid expressing pcDNA3.1 (+) / ChM-I was constructed, and transfected into MSCs. Stable expression verified that ChM-I is upregulated in MSCs, which make it a useful preparation for studying the further role of ChM-I in inducing bone MSCs toward a chondrogenic I phenotype and for tissue engineering constructs. MSCs were obtained from adult Sprague Dawley (SD) rats using density gradient centrifugation. Cell surface antigens were detected by flow cytometry. The experimental groups were MSCs transfected with the plasmid pcDNA3.1 (+)/ChM-I. The control one was with the plasmid pcDNA3.1 (+) and the negative control one was with no plasmid. Transcription and translation were detected in the level of mRNA and protein respectively in order to identify the stability of the expression of ChM-I in cell lines by RT-PCR and western blot analysis. The results of RT-PCR showed that the mRNA level of ChM-I in the experimental group was six times higher than the control. The quantitative optical density analysis showed that the expression of ChM-I in the experimental group was significantly higher than the control by western blot. We conclude that we have established MSCs lines stably expressing ChM-I. This work lays the foundation of studying the potential role of the ChM-I in inducing BMSCs into chondrocytes.

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RNA-seq of H1299 cells stably transfected with empty vector or the p53 mutant R273H.

Project description:This experiment aimed to determine the genes that were upregulated when mutant p53 (R273H) was expressed. We used H1299 lung cancer cells that are endogenously p53-null and overexpressed the p53 mutant.

2017-04-16 | E-MTAB-5652 | biostudies-arrayexpress

ChIP-seq of H1299 cells stably transfected with empty vector or the p53 mutant R273H

Project description:This experiment was performed to determine which gene promoters mutant p53 binds and transcriptionally regulates in order to understand how mutant p53 accomplishes its gain of function phenotype.

2017-04-16 | E-MTAB-5653 | biostudies-arrayexpress

RNA-seq of MCF10A cells stably expressing empty vector, GFP, miR-105, or MYC

Project description:To identify gene expression changes associated with overexpression of miR-105 or MYC in MCF10A non-cancerous human mammary epithelial cells, we analyzed RNA isolated from engineered MCF10A cell lines that stably express empty vector, GFP, miR-105, or MYC by RNA-seq. Gene expression in cells overexpressing miR-105 or MYC was compared to cells expressing the empty vector or GFP, both of which served as controls in this experiment.

2018-02-11 | GSE106502 | GEO

Expression data for nasopharyngeal carcinoma cell line 5-8F stably expressing either LTF gene or empty vector.

2013-12-26 | GSE36972 | GEO

Expression data of NCI-H441 cells stably expressing hsa-mir-365-2 vs empty vector

2011-11-15 | GSE33672 | GEO

Expression data for nasopharyngeal carcinoma cell line 5-8F stably expressing either LTF gene or empty vector.

Project description:LTF (lactotransferrin, lactoferrin) is a key component of innate immune defense. It has recently been found to have antitumor and antimetastatic activity in different cancers. We previously reported LTF to be the most significantly downregulated gene in nasopharyngeal carcinoma (NPC) specimens relative to normal nasopharyngeal epithelial tissues and it was also negatively associated with the progression and metastasis of NPC. However, the mechanism underlying this remains unclear. In the current study, we performed genome expression profiling analysis using the Affymetrix genome wide microarray system to identify broad scale changes in gene expression associated with LTF gene overexpression in NPC cell line 5-8F. NPC cell line5-8F were transfected with either PIRES empty vector (Clontech) or PIRES-LTF vector by Lipofectamine 2000 (Invitrogen). Cells stably expressing either LTF or empty vector were generated by selection with growth medium supplemented with 400 M-NM-<g/mL G418. When cells were growing at 80% confluence, total RNA were extracted and detected by Affymerix GeneChip Human Gene U133 plus2.0.

2013-12-26 | E-GEOD-36972 | biostudies-arrayexpress

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