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HITS-CLIP reveals key regulators of nuclear receptor signaling in breast cancer

ABSTRACT: We used a genome-wide approach (High-throughput sequencing of RNA isolated by crosslinking immunoprecipitation, or HITS-CLIP) to define direct miRNA-mRNA interactions in three breast cancer subtypes (estrogen receptor positive, Her2 amplified and triple negative). Focusing on steroid receptor signaling, we identified two novel regulators of the ER pathway (miR-9-5p and miR-193a/b-3p), which together target multiple genes involved in ER signaling. Moreover, this approach enabled the definition of miR-9-5p as a global regulator of steroid receptor signaling in breast cancer. Finally, we show that miRNA targets and networks defined by our analysis are predictive of patient outcomes and provide global insight into miRNA regulation in breast cancer. Argonaute HITS-CLIP on three representative breast cancer cell lines (each in triplicate).

ORGANISM(S): Homo sapiens

SUBMITTER: Pillai MM

PROVIDER: S-ECPF-GEOD-57855 | biostudies-other | 2014 Jul

REPOSITORIES: biostudies-other

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HITS-CLIP reveals key regulators of nuclear receptor signaling in breast cancer.

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