Transcription profiling of human pancreas NS-928 cell lines modulated by COX-2

ABSTRACT: BxPc-3, Miapaca-2 and ASPC-1 cell lines were treated with 100 µM NS-398 in DMSO or with DMSO alone (control) for 48 h. Total-RNA from each sample was isolated with the RNeasy kit of Qiagen (Hilden, Germany) according to the manufacturer's protocol. The integrity of the isolated RNA was checked on an Agilent Bioanalyser 2100 using the RNA 6000 Nano Kit (Agilent Technologies, Palo Alto, USA) according to the manufacturer's protocol as recommended by the manufacturer.
Microarrays were produced and processed as described before. Human cDNAs representing some 7,000 genes that are highly associated with the occurrence of pancreatic cancer – including apoptotic and oncogenic genes, growth factors, angiogenic, cell cycle, metastasis-associated, and housekeeping genes – were PCR-amplified using

ORGANISM(S): Homo sapiens

SUBMITTER: Youns Mahmoud 

PROVIDER: S-ECPF-MEXP-1870 | biostudies-other |

REPOSITORIES: biostudies-other