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Transcription profiling of human lung adenocarcinoma derived cell line A549 treated with testosterone

ABSTRACT: Effect of testosterone treatment on gene expression of lung adenocarcinoma derived cell line A549

ORGANISM(S): Homo sapiens

SUBMITTER: Mikkonen Laura

PROVIDER: S-ECPF-MEXP-2003 | biostudies-other |

REPOSITORIES: biostudies-other

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Transcription profiling of human lung adenocarcinoma derived cell line A549 treated with dexamethasone

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Transcription profiling of human lung adenocarcinoma derived cell line A549 treated with testosterone

Project description:Effect of testosterone treatment on gene expression of lung adenocarcinoma derived cell line A549

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Transcription profiling of human A549 lung adenocarcinoma cell line time Course of TGF-beta treatment

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Transcription profiling of human lung adenocarcinoma derived cell line A549 treated with dexamethasone

Project description:Effect of dexamethasone on gene expression in lung adenocarcinoma derived cell line A549

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Noncoding RNA expression profiling of cisplatin-resistant cells derived from the A549 lung cell line

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

| S-ECPF-GEOD-43494 | biostudies-other

Noncoding RNA expression profiling of cisplatin-resistant cells derived from the A549 lung cell line (miRNA)

Project description:To determine the signaling networks that are dysregulated in cisplatin-resistant non-small cell lung cancer, noncoding RNA expression data were obtained from, and compared between, the lung adenocarcinoma cell line, A549, and its cisplatin-resistant derivative, A549/CDDP. Noncoding RNA expression data from a cisplatin-sensitive lung adenocarcinoma cancer cell line (A549) were collected and compared to noncoding RNA expression data from a cisplatin-resistant cell line (A549/CDDP). 3 independent experiments were completed for both the sensitive and resistant cell lines.

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Transforming growth factor-? impairs glucocorticoid activity in the A549 lung adenocarcinoma cell line.

Project description:Background and purposeThe lung adenocarcinoma cell line, A549, undergoes epithelial-mesenchymal cell transition (EMT) in response to TGF-?. Glucocorticoids do not prevent the EMT response, but TGF-? induced resistance to the cytokine-regulatory action of glucocorticoids. We sought to characterize the impairment of glucocorticoid response in A549 cells.Experimental approachA549 cells were exposed to TGF-? for up to 96?h before glucocorticoid treatment and challenge with IL-1? to assess glucocorticoid regulation of IL-6 and CXCL8 production. Nuclear localization of the glucocorticoid receptor ? (GR?) was ascertained by immunofluorescence and Western blotting. Transactivation of the glucocorticoid response element (GRE) was measured with a transfected GRE-secreted human placental alkaline phosphatase reporter.Key resultsTGF-? (40-400?pM) reduced the maximum inhibitory effect of dexamethasone on IL-1?-induced IL-6 and CXCL8 production. The impaired glucocorticoid response was detected with 4?h of TGF-? (40?pM) exposure (and 4?h IL-1? to induce CXCL8 expression) and therefore was not secondary to EMT, a process that requires longer incubation periods and higher concentrations of TGF-?. TGF-? also impaired dexamethasone regulation of granulocyte-macrophage colony-stimulating factor in thrombin-stimulated BEAS-2B epithelial cells. Impaired regulation of CXCL8 was associated with markedly reduced GRE transactivation and reduced induction of mRNA for I?B?, the glucocorticoid-inducible leucine zipper and the epithelial sodium channel (SCNN1A). The expression, cellular levels and nuclear localization of GR? were reduced by TGF-?.Conclusions and implicationsWe have identified mechanisms underlying the impairment of responses to glucocorticoids by TGF-? in the A549 and BEAS-2B cell lines.

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DUS4L Silencing Suppresses Cell Proliferation and Promotes Apoptosis in Human Lung Adenocarcinoma Cell Line A549.

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Gene expression profiling of cancer stem cell in human lung adenocarcinoma A549 cells.

Project description:BACKGROUND: The studies on cancer-stem-cells (CSCs) have attracted so much attention in recent years as possible therapeutic implications. This study was carried out to investigate the gene expression profile of CSCs in human lung adenocarcinoma A549 cells. RESULTS: We isolated CSCs from A549 cell line of which side population (SP) phenotype revealed several stem cell properties. After staining the cell line with Hoechst 33342 dye, the SP and non-side population (non-SP) cells were sorted using flow cytometric analysis. The mRNA expression profiles were measured using an Affymetrix GeneChip(R) oligonucleotide array. Among the sixty one differentially expressed genes, the twelve genes inclusive three poor prognostic genes; Aldo-keto reductase family 1, member C1/C2 (AKR1C1/C2), Transmembrane 4 L six family member 1 nuclear receptor (TM4SF1), and Nuclear receptor subfamily 0, group B, member 1 (NR0B1) were significantly up-regulated in SP compared to non-SP cells. CONCLUSION: This is the first report indicating the differences of gene expression pattern between SP and non-SP cells in A549 cells. We suggest that the up-regulations of the genes AKR1C1/C2, TM4SF1 and NR0B1 in SP of human adenocarcinoma A549 cells could be a target of poor prognosis in anti-cancer therapy.

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