Project description:We have investigated the effect of exposure to 150 mg/kg benzo(a)pyrene (BaP) for 3 days on mRNA and miRNA expression levels in adult mouse liver. We used Agilent miRNA array platforms to assess effects of BaP exposure on miRNA expression levels. Our results indicate a distinct lack of effect of BaP of miRNA expression, despite widespread changes in mRNA levels. The data in the attached array files were used a positive control for the Agilent platform, to indicate that the platform was able to detect significant differences in abundance of miRNA between two samples with great differences in miRNA content. Keywords: Toxicology, miRNA

2010-10-28 | GSE19438 | GEO

MicroRNA-145 Regulates Human Corneal Epithelial Differentiation [Agilent-016436 array data]

Project description:To investigate the microRNA expression in human limbal-peripheral corneal (LPC) epithelia containing corneal epithelial progenitor cells (CEPCs) and early transit amplifying cells, we have employed Human microRNA Microarray V2 (Agilent) as a screening platform to identify specific microRNAs. We discovered a differential expression of 18 microRNAs against central corneal (CC) epithelia, which contains late transit amplifying cells and terminally differentiated cells. Among them, cluster miR-143/145 was expressed strongly in LPC but at low levels in CC epithelia and this was validated by real-time PCR and locked nucleic acid-based in situ hybridization.

2011-10-10 | GSE24979 | GEO

MicroRNA-145 Regulates Human Corneal Epithelial Differentiation [Agilent-014850 array data]

Project description:To investigate the gene expression in human corneal epithelial overexpressing hsa-miR-145 by transfection , we have employed Whole Human Genome Oligo Microarray (Agilent) as a screening platform to identify gene regulation. We discovered a differential gene expression in HCE cells transfected with hsa-mIR-145 against cells with scrambled sequences. Among them, genes related with corneal development, integrity, differentiation and inflammatory responses were found and this was validated by real-time PCR.

2011-10-10 | GSE24980 | GEO

caArray_topal-00039: TCGA (GBM): MicroRNA Analysis of TCGA GBM samples using Agilent MicroRNA array (UNC)

Project description:MicroRNA Analysis of TCGA GBM samples using Agilent MicroRNA array

2016-12-09 | GSE91014 | GEO

Evaluating annotations of an Agilent expression chip suggests that many features cannot be interpreted.

Project description: Not available

| S-EPMC2791105 | biostudies-literature