Unknown

Dataset Information

0

Role of protein kinase C in the acrosome reaction of mammalian spermatozoa.


ABSTRACT: Mammalian spermatozoa undergo a Ca(2+)-dependent exocytotic event before fertilization which is known as the acrosome reaction. The process of exocytosis in several cell systems is mediated by a protein kinase C (PKC)-catalysed phosphorylation. Addition of phorbol 12-myristate-13-acetate or the membrane-permeant diacylglycerol analogue 1-oleoyl-2-acetylglycerol, which are potent activators of PKC, to bovine spermatozoa resulted in stimulation of the acrosome reaction. This stimulation was inhibited by low concentrations (50% inhibition at 0.7 nM) of the PKC inhibitor staurosporine. PKC specific activity in bovine spermatozoa is extremely low in comparison with other cells; however, it is comparable with the activity found in human spermatozoa. Immunohistochemical analysis using anti-PKC antibodies revealed staining in the equatorial segment, the post-acrosomal region and the upper region of the head. We propose that PKC is involved in the mammalian sperm acrosome reaction.

SUBMITTER: Breitbart H 

PROVIDER: S-EPMC1130709 | biostudies-other | 1992 Jan

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC3952862 | biostudies-other
| S-EPMC1138523 | biostudies-other
| S-EPMC1151443 | biostudies-other
| S-EPMC19685 | biostudies-literature
| S-EPMC8614796 | biostudies-literature
| S-EPMC3064341 | biostudies-literature
| S-EPMC6878212 | biostudies-literature
| S-EPMC3739068 | biostudies-other
| S-EPMC8328089 | biostudies-literature
| S-EPMC8122410 | biostudies-literature