Unknown

Dataset Information

0

Immunological localization of ribosomes in striated rat muscle. Evidence for myofibrillar association and ontological changes in the subsarcolemmal:myofibrillar distribution.


ABSTRACT: Ribosome distribution in skeletal-muscle fibres was investigated immunohistochemically by using polyclonal antibodies raised against large-ribosomal-subunit proteins isolated from rat liver. Immunoblot analysis showed the antibodies to recognize five major proteins of the large subunit; these were identified as L4, L6, L7, L15 and L17 by two-dimensional electrophoresis. Immunohistochemistry of frozen rat skeletal-muscle sections showed staining of both the subsarcolemmal and intermyofibrillar cytoplasm. A distinct banding pattern was observed, and when peroxidase and phase-contrast images of the same field were compared by image analysis the anti-ribosome staining was found to correspond to the A-bands. These results suggest that a proportion of muscle ribosomes are present in the myofibrillar cytoplasm in a regular fashion, possibly associated with myosin. Densitometric analysis of the peroxidase immunostaining showed that the ratio of myofibrillar to sub-sarcolemmal ribosomal material was lower in muscle from 51-day-old rats compared with those from 14-day-old animals.

SUBMITTER: Horne Z 

PROVIDER: S-EPMC1131417 | biostudies-other | 1990 May

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC8511094 | biostudies-literature
| S-EPMC7429545 | biostudies-literature
| S-EPMC4620896 | biostudies-literature
| S-EPMC7693451 | biostudies-literature
| S-EPMC2583436 | biostudies-literature
| S-EPMC6519837 | biostudies-literature
| S-EPMC4415482 | biostudies-literature
| S-EPMC9827075 | biostudies-literature
| S-EPMC6218675 | biostudies-literature
| S-EPMC6117265 | biostudies-literature