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Measurement of 5-aminolaevulinate synthase activity by gas-liquid chromatography with electron-capture detection.


ABSTRACT: A specific assay for 5-aminolaevulinate synthase activity is described, with a sensitivity comparable with that of radiochemical assays. It is based on measurement by g.l.c. with electron-capture detection of the pentafluorobenzyl ester of the ethyl acetoacetate pyrrole derivative of 5-aminolaevulinic acid, and of the corresponding compound from 6-amino-5-oxohexanoic acid used as internal standard. Enzyme activity has been measured in homogenates of rat liver, spleen, kidney and brain, and in human lymphocytes.

SUBMITTER: Gorchein A 

PROVIDER: S-EPMC1131684 | biostudies-other | 1990 Aug

REPOSITORIES: biostudies-other

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