Unknown

Dataset Information

0

Sequence similarity of calreticulin with a Ca2(+)-binding protein that co-purifies with an Ins(1,4,5)P3-sensitive Ca2+ store in HL-60 cells.


ABSTRACT: HL-60 cells possess a 60 kDa Ca2(+)-binding protein that is contained in a discrete subcellular compartment, referred to as calciosomes. Subcellular fractionation studies have suggested that, in HL-60 cells, this intracellular compartment is an Ins(1,4,5)P3-sensitive Ca2+ store. In order to investigate the structural relationship of the 60 kDa Ca2(+)-binding protein of HL-60 cells to other Ca2(+)-binding proteins, we have purified the protein by ammonium sulphate extraction, acid precipitation, and DEAE-cellulose and phenyl-Sepharose column chromatography. The N-terminal sequence of the protein shows 93% identity with rabbit muscle calreticulin, a recently cloned sarcoplasmic reticulum Ca2(+)-binding protein. No amino acid sequence similarity with calsequestrin was found, although the purified protein cross-reacted with anti-calsequestrin antibodies. The calreticulin-related protein of HL-60 cells might play a role as an intravesicular Ca2(+)-binding protein of an Ins(1,4,5)P3-sensitive Ca2+ store.

SUBMITTER: Krause KH 

PROVIDER: S-EPMC1131758 | biostudies-other | 1990 Sep

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC1136288 | biostudies-other
| S-EPMC3327615 | biostudies-literature
| S-EPMC1137203 | biostudies-other
| S-EPMC1220408 | biostudies-other
| S-EPMC3603520 | biostudies-literature
| S-EPMC1131438 | biostudies-other
| S-EPMC2782698 | biostudies-literature
| S-EPMC1217874 | biostudies-other
| S-EPMC1133948 | biostudies-literature
| S-EPMC5655508 | biostudies-literature