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Hyaluronate synthase: cloning and sequencing of the gene from Streptococcus sp.


ABSTRACT: The complete nucleotide sequence of hyaluronate synthase from Streptococcus sp. and its flanking regions is presented. The gene locus was designated has. Southern-blotting results suggested that the gene was conserved in hyaluronate-producing streptococci. A putative translation-initiation codon was identified and the open reading frame consists of 1566 bp, specifying a protein of 56 kDa. Sequences resembling the promoter and ribosome-binding site of Gram-positive organisms are found upstream of the synthase. The predicted amino-acid sequence reveals the presence of a 35-residue signal peptide. The sequence has some similarity to bacterial peptide-binding proteins.

SUBMITTER: Lansing M 

PROVIDER: S-EPMC1132147 | biostudies-other | 1993 Jan

REPOSITORIES: biostudies-other

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