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Identification of a novel autophosphorylation site (P4) on the epidermal growth factor receptor.


ABSTRACT: Three major autophosphorylation sites are located near the C-terminus of the epidermal growth factor receptor, but a fourth site is repeatedly detected. We report here the purification and sequencing of a tryptic peptide containing this site, Tyr-1086. Furthermore, we demonstrate that additional phosphopeptides are observed following both partial digestion and overdigestion. Finally, we show that Tyr-1086 can be phosphorylated in intact cells.

SUBMITTER: Hsuan JJ 

PROVIDER: S-EPMC1133318 | biostudies-other | 1989 Sep

REPOSITORIES: biostudies-other

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