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Purification of TEM-1 beta-lactamase by immunoaffinity chromatography.


ABSTRACT: A monoclonal antibody prepared against TEM-1 beta-lactamase was found to compete with penicillins and cephalosporins for binding to the enzyme. The purified antibody preparation was linked to Sepharose 4B and used for immunoaffinity-chromatography purification of TEM-1 beta-lactamase. Elution with either benzylpenicillin or cloxacillin yielded a highly purified, concentrated and active enzyme preparation.

SUBMITTER: Bibi E 

PROVIDER: S-EPMC1133427 | biostudies-other | 1989 Oct

REPOSITORIES: biostudies-other

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