Unknown

Dataset Information

0

Chorismate synthase. Pre-steady-state kinetics of phosphate release from 5-enolpyruvylshikimate 3-phosphate.

ABSTRACT: The pre-steady-state kinetics of phosphate formation from 5-enolpyruvylshikimate 3-phosphate catalysed by Escherichia coli chorismate synthase (EC 4.6.1.4) were studied by a rapid-acid-quench technique at 25 degrees C at pH 7.5. No pre-steady-state 'burst' or 'lag' phase was observed, showing that phosphate is released concomitant with the rate-limiting step of the enzyme. The implications of this result for the mechanism of action of chorismate synthase are discussed.

SUBMITTER: Hawkes TR 

PROVIDER: S-EPMC1133717 | biostudies-other | 1990 Feb

REPOSITORIES: biostudies-other

Json Xml

Similar Datasets

Unknown Pre-steady-state kinetics for hydrolysis of insoluble cellulose by cellobiohydrolase Cel7A.
| S-EPMC3365755 | biostudies-literature
Unknown DNA polymerase fidelity: comparing direct competition of right and wrong dNTP substrates with steady state and pre-steady state kinetics.
| S-EPMC2803633 | biostudies-literature
Unknown A steady-state and pre-steady-state kinetics study of the tungstoenzyme formaldehyde ferredoxin oxidoreductase from Pyrococcus furiosus.
| S-EPMC2099461 | biostudies-other
Unknown Inhibition of Inosine-5'-monophosphate Dehydrogenase from Bacillus anthracis: Mechanism Revealed by Pre-Steady-State Kinetics.
| S-EPMC5524190 | biostudies-literature
Unknown Steady-state and pre-steady-state kinetic analysis of Mycobacterium smegmatis cysteine ligase (MshC).
| S-EPMC2526253 | biostudies-literature
Unknown The mechanism of Klebsiella pneumoniae nitrogenase action. Pre-steady-state kinetics of H2 formation.
| S-EPMC1144524 | biostudies-other
Transcriptomics Adaptive laboratory evolution of chorismate synthase knockouts
2023-02-08 | GSE224889 | GEO
Unknown Electrogenic glutamate transporters in the CNS: molecular mechanism, pre-steady-state kinetics, and their impact on synaptic signaling.
| S-EPMC2389879 | biostudies-literature
Unknown Pre-steady-state decoding of the Bicoid morphogen gradient.
| S-EPMC1790957 | biostudies-literature
Unknown Acyl-CoA subunit selectivity in the pikromycin polyketide synthase PikAIV: steady-state kinetics and active-site occupancy analysis by FTICR-MS.
| S-EPMC3184853 | biostudies-literature