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Lack of association of G-protein beta 2- and gamma 2-subunit N-terminal fragments provides evidence against the coiled-coil model of subunit-beta gamma assembly.


ABSTRACT: The association between peptides from bovine G-protein beta 2- and gamma 2-subunits was studied by CD spectroscopy and cross-linking. Both peptides had approximately 25% stable alpha-helical structure at 25 degrees C, but there was no increase on mixing subunits as expected for coiled-coil formation. Also, disulphide cross-linking gave more beta 2 beta 2 homodimer than beta 2 gamma 2 heterodimer. These data do not support the proposed N-terminal coiled-coil model of beta gamma-subunit association.

SUBMITTER: Marin EP 

PROVIDER: S-EPMC1135741 | biostudies-other | 1995 Jul

REPOSITORIES: biostudies-other

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