Unknown

Dataset Information

0

Structural analysis of inositol phospholipids from Trypanosoma cruzi epimastigote forms.


ABSTRACT: Inositol phospholipids (IPL) from epimastigote forms of Trypanosoma cruzi have been investigated by metabolic labelling with [3H]palmitic acid and by GLC-MS analysis of the lipids obtained from non-labelled parasites. The IPL fraction was separated into phosphatidylinositol (PI) and inositol-phosphoceramide subfractions, the latter accounting for 80-85% of the total IPL. The neutral lipids released from the IPLs by PI-specific phospholipase C (PI-PLC) from Bacillus thuringiensis were analysed by silica-gel and reverse-phase TLC for the radioactive lipids and by GLC-MS for the non-radioactive samples. Ceramides containing dihydrosphingosine and sphingosine with C16:0 and C18:0 fatty acids were identified. The main component in the [3H]palmitic acid-labelled ceramides was palmitoyldihydrospingosine, while in the non-labelled sample the ceramides contained mainly sphingosine. This could reflect partial uptake of phospholipid from the medium. The PI contain both alkylacyl- and diacyl-glycerol lipids, with the ether lipid being more abundant. The latter was identified as 1-O-hexadecylglycerol esterified by C18:2 and C18:1 fatty acids. Interestingly, the same lipid had been identified in the anchor of the 1G7 glycoprotein of T. cruzi metacyclic forms.

SUBMITTER: Bertello LE 

PROVIDER: S-EPMC1135881 | biostudies-other | 1995 Aug

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC10466500 | biostudies-literature
| S-EPMC1134981 | biostudies-literature
| S-EPMC2752740 | biostudies-literature
| S-EPMC7864430 | biostudies-literature
| S-EPMC4125143 | biostudies-literature
| S-EPMC1150068 | biostudies-other
| S-EPMC8879499 | biostudies-literature
| S-EPMC4970754 | biostudies-literature
| S-EPMC5217780 | biostudies-literature
2023-04-11 | GSE229354 | GEO