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Prolactin increases hepatic Na+/taurocholate co-transport activity and messenger RNA post partum.

ABSTRACT: We have shown that Na+/taurocholate co-transport activity is decreased in pregnancy, but rebounds post partum relative to non-pregnant controls, and that activity can be increased by treatment with ovine prolactin [Ganguly, Hyde and Vore (1993) J. Pharmacol. Exp. Ther. 267, 82-87]. To determine the basis for these effects, Na+/taurocholate co-transport was determined in purified basolateral liver plasma-membrane (bLPM) vesicles and compared with steady-state mRNA levels encoding the Na+/taurocholate-co-transporting polypeptide (Ntcp) in non-pregnant controls, pregnant rats (19-20 days pregnant), rats post partum (48 h post partum) and rats post partum treated with bromocriptine to inhibit prolactin secretion. Na+/taurocholate co-transport activity (nmol/5 s per mg of protein) in bLPM was decreased from 10.4 +/- 1.8 in non-pregnant controls to 7.9 +/- 0.6 in bLPM in pregnant rats, but rebounded to 17.5 +/- 1.3 post partum; treatment of rats post partum with bromocriptine to inhibit prolactin secretion decreased activity to 14.1 +/- 0.9. Northern and slot-blot analyses revealed similar changes in mRNA for Ntcp, so that a positive correlation was observed between Na+/taurocholate co-transport activity and Ntcp mRNA. Furthermore, treatment of ovariectomized rats with ovine prolactin increased Ntcp mRNA 10-fold compared with solvent-treated controls, consistent with the 2-fold increase in Vmax, for Na+/taurocholate co-transport in isolated hepatocytes. These data are the first to demonstrate endogenous physiological regulation by prolactin of Ntcp mRNA in parallel with Na+/taurocholate co-transport activity.

SUBMITTER: Ganguly TC

PROVIDER: S-EPMC1137552 | biostudies-other | 1994 Oct

REPOSITORIES: biostudies-other

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Project description:BACKGROUND:This study aims to explore the difference in the utilization pattern of dental services among pregnant, post-partum and six-month post-partum women. METHODS:This cross-sectional questionnaire survey was performed at two maternity and child care hospitals in India that primarily cater to middle and low income communities. Data were collected from 3 groups: 1) pregnant women in their first trimester; 2) post-partum women (<?48?h after delivery); and 3) six-month post-partum women. The primary outcome of interest was dental service utilization during pregnancy. Self-perceived oral health (SPOH) was calculated based on the four global dimensions- knowledge, function, quality of life and social. Multiple logistic regression analysis was carried out to assess the effect of each independent variable after adjustment for the effect of all other variables in the model. RESULTS:Responses of 450 (150 pregnant, 150 post-partum and 150 six-month post-partum) women were analyzed (response rate?=?72%). Significant differences in the dental attendance pattern was observed between the study groups (p?<?0.01). Dental attendance among pregnant and six-month post-partum women were 60 and 75%, respectively, however, only about 15% of the post-partum women reported to have sought dental care within the 6?months prior to the study. Post-partum women had the highest SPOH scores, indicating poor self-perceived oral health, followed by pregnant and then six-month post-partum women, which was statistically significant (p?< 0.05). A significantly higher percentage of post-partum women reported to have poor oral and general health, as compared to both, pregnant and six-month post-partum women (p?< 0.01). Higher percentage of women reporting 'good' oral and general health had sought dental care compared with others (p?< 0.01). After adjusting for all the other variables in the model, women with lower levels of education (ORa?=?1.42; 95% CI: 1.01-2.00), women with poor self-perceived oral health (ORa?=?1.08; 95% CI: 1.02-1.14) and post-partum women (ORa?=?0.15; 95% CI: 0.09-0.24) were found to be less likely to seek regular dental care. CONCLUSION:Pattern of dental service utilization among women in this population varied according to their pregnancy status, level of education and self-perceived oral health.

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Prolactin increases hepatic Na+/taurocholate co-transport activity and messenger RNA post partum.

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