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Lipopolysaccharide-induced change of ADP-ribosylation of a cytosolic protein in bone-marrow-derived macrophages.


ABSTRACT: Treatment of bone-marrow-derived macrophages with nanogram quantities of bacterial lipopolysaccharide (LPS) or with the synthetic bacterial lipopeptide analogue N-palmitoyl-(S)-[2,3-bis(palmitoyloxy)-(2RS)-propyl] (Pam3)Cys-Ala-Gly results in a change of ADP-ribosylation of a cytosolic 33 kDa protein. The immunostimulant-induced change is both dose- and time-dependent. It is not observed in macrophages from an LPS-unresponsive C3H/HeJ mouse strain upon treatment with LPS. Non-endotoxic LPS from Rhodopseudomonas pallustris, the inactive lipopeptide analogue Pam3CysOH, and LPS in the presence of polymyxin B fail to induce the change of ADP-ribosylation of the protein. These observations indicate that reversible protein modification by ADP-ribosylation might play a role in macrophage activation.

SUBMITTER: Hauschildt S 

PROVIDER: S-EPMC1137782 | biostudies-other | 1994 Jan

REPOSITORIES: biostudies-other

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