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Comparison of different techniques for estimating rates of protein synthesis in vivo in healthy and bacteraemic rats.

ABSTRACT: Previous studies have reported that use of a flooding dose of radiolabelled amino acid is a more precise technique than the constant infusion of tracer quantities for determining rates of protein synthesis in rapidly turning-over tissues in the rat. However, there has been little direct investigation comparing different methods under comparable conditions. Initially, 12 healthy male Sprague-Dawley rats, weighing approx. 100 g, were randomized to receive either a bolus intravenous injection of 100 mumol of L-leucine (containing 30 microCi of [1-14C]leucine)/100 g body wt., or a continuous 2 h tracer infusion of [14C]leucine. In the second phase of the experiment, 12 additional rats were intravenously injected with 1 X 10(8) colony-forming units of Pseudomonas aeruginosa and 16 h later randomized to receive one of two infusions described above. Total protein synthesis as well as fractional synthesis rates were determined in liver, rectus muscle and whole body. Synthesis rates measured in liver, muscle and whole body were significantly higher in bacteraemic rats than in healthy rats. The flooding-dose methodology gave significantly higher estimates of protein synthesis in the liver, skeletal muscle and whole body than did the continuous-infusion method using direct measurement of the acid-soluble fraction from the respective tissue. Indirect estimates of whole-body protein synthesis based on plasma enrichments and stochastic modelling gave the lowest values.

SUBMITTER: Pomposelli JJ

PROVIDER: S-EPMC1144674 | biostudies-other | 1985 Feb

REPOSITORIES: biostudies-other

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NMR Based Metabolomics Comparison of Different Blood Sampling Techniques in Awake and Anesthetized Rats.

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Absolute quantification of protein production reveals principles underlying protein synthesis rates

2014-04-24 | GSE53767 | GEO

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| S-EPMC10280615 | biostudies-literature

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