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High-performance liquid chromatography of uroporphyrinogen and coproporphyrinogen isomers with amperometric detection.

ABSTRACT: A reversed-phase h.p.l.c. system, with an ODS-Hypersil column with acetonitrile or methanol in ammonium acetate buffer as mobile phase, is described for the separation of uro-and copro-porphyrinogen isomers. The porphyrinogens are detected amperometrically with sensitivity comparable with that of the fluorescent detection of porphyrins. The effects of pH, buffer concentration and organic modifiers on retention and resolution were studied. The method is suitable for both analytical and preparative separation of porphyrinogens.

SUBMITTER: Lim CK

PROVIDER: S-EPMC1146618 | biostudies-other | 1986 Mar

REPOSITORIES: biostudies-other

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High-performance liquid chromatography of coproporphyrin isomers.

Project description:A reversed-phase system is described for the simultaneous isocratic separation of coproporphyrin I, II, III and IV isomers. The retention behaviour of coproporphyrin I and III is studied in detail. The method is suitable for both analytical and semi-preparative separation.

| S-EPMC1154125 | biostudies-other

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| S-EPMC1133374 | biostudies-other

Separation and characterization of pentacarboxylic porphyrinogen isomers by high-performance liquid chromatography with electrochemical detection.

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| S-EPMC2780435 | biostudies-literature

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| S-EPMC7322751 | biostudies-literature

Quick Test for Determination of N-Bombs (Phenethylamine Derivatives, NBOMe) Using High-Performance Liquid Chromatography: A Comparison between Photodiode Array and Amperometric Detection.

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| S-EPMC6740171 | biostudies-literature

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| S-EPMC1152093 | biostudies-other

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