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Purification of the constituent enzyme fractions of mycobacillin synthetase.

ABSTRACT: The final purification of the three-fraction enzyme complex mycobacillin synthetase was done by hydroxyapatite column chromatography and sucrose-density-gradient centrifugation; each of the fractions obtained migrates as a single component in SDS/polyacrylamide-gel electrophoresis and gel electrofocusing. The Mr of the enzyme fractions A, B and C by gel filtration is 260 000, 190 000 and 105 000, and that by SDS/polyacrylamide-gel electrophoresis is 252 000, 198 000 and 108 000 respectively. None of the enzyme fractions appears to possess subunit structure.

SUBMITTER: Ghosh SK

PROVIDER: S-EPMC1146651 | biostudies-other | 1986 Apr

REPOSITORIES: biostudies-other

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Functional characterization of constituent enzyme fractions of mycobacillin synthetase.

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