Unknown

Dataset Information

0

Fractionation of rat liver plasma-membrane regions by two-phase partitioning.


ABSTRACT: Rat liver plasma membranes, enriched in blood-sinusoidal or bile-canalicular regions by differential and sucrose-gradient centrifugation, were further purified by partitioning in an aqueous polymer two-phase system. This method separates membranes according to differences in surface properties rather than size and density. A several-fold increase in the ratio of leucine aminopeptidase (a bile-canalicular marker) and 5'-nucleotidase to asialo-orosomucoid binding (a blood-sinusoidal marker) was obtained in one fraction, whereas another fraction gave a 2-3-fold increase in ratio of blood-sinusoidal to bile-canalicular markers. Furthermore, the markers for both regions of the plasma membrane, as well as markers for Golgi membranes and lysosomes, showed a heterogeneous behaviour on counter-current distribution.

SUBMITTER: Gierow P 

PROVIDER: S-EPMC1146742 | biostudies-other | 1986 May

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC1133228 | biostudies-other
| S-EPMC2908281 | biostudies-literature
| S-EPMC1146673 | biostudies-other
| S-EPMC2481404 | biostudies-literature
| S-EPMC1153278 | biostudies-other
| S-EPMC6368448 | biostudies-literature
| S-EPMC1149895 | biostudies-other
| S-EPMC10547480 | biostudies-literature
| S-EPMC3853087 | biostudies-other
| S-EPMC3840720 | biostudies-literature