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Pig heart fumarase really does exhibit negative kinetic co-operativity at a constant ionic strength.


ABSTRACT: The kinetics of the action of fumarase on L-malate and fumarate were investigated at constant ionic strength. This was done to evaluate reports that fumarase follows simple Michaelis-Menten kinetics. However, when pH, buffer concentration and ionic strength are all maintained at constant values, the Lineweaver-Burk plots exhibit pronounced downward curvature, characteristic of negative kinetic co-operativity.

SUBMITTER: Hasinoff BB 

PROVIDER: S-EPMC1146771 | biostudies-other | 1986 May

REPOSITORIES: biostudies-other

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