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Studies on the biotin-binding site of avidin. Lysine residues involved in the active site.

ABSTRACT: Egg-white avidin was treated with 1-fluoro-2,4-dinitrobenzene. Modification of an average of one lysine residue per avidin subunit caused the complete loss of biotin binding. Tryptic peptides obtained from the 2,4-dinitrophenylated avidin were fractionated by reversed-phase h.p.l.c. Three peptides contained the 2,4-dinitrophenyl group. Amino acid analysis revealed that lysine residues 45, 94 and 111 are modified and probably comprise part of the biotin-binding site.

SUBMITTER: Gitlin G 

PROVIDER: S-EPMC1147797 | biostudies-other | 1987 Mar

REPOSITORIES: biostudies-other

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