A residence-time analysis of enzyme kinetics.

ABSTRACT: A 'first-passage-time' analysis is applied to enzyme kinetics. It is shown that the residence times determined in this way are directly related to the steady-state parameters and are particularly useful in analysis of isotopic exchange. A simple linear means is used for the calculation of these residence times that makes this method easily applicable to the numerical evaluation of complex models. This stochastic type of approach provides an alternative that avoids the classical steady-state approximation that the concentrations of enzyme intermediates are constant. Instead, steady state is defined as the randomization of the states of the enzyme following initial mixing due to completion of the turnovers of individual enzyme molecules at different times.

SUBMITTER: Sines JJ 

PROVIDER: S-EPMC1147827 | biostudies-other | 1987 Apr

REPOSITORIES: biostudies-other