Unknown

Dataset Information

0

A developmentally controlled change in the post-translational modifications on the lysosomal alpha-mannosidase of the cellular slime mould Dictyostelium discoideum.


ABSTRACT: During the development of the cellular slime mould Dictyostelium discoideum, a second form of a number of lysosomal enzymes begins to accumulate. The second ('late') form of these enzymes differs from the pre-existing ('early') form in post-translational modification. Pulse-chase experiments using [35S]methionine show that the late form of alpha-mannosidase-1 is made by synthesis de novo starting 8 h after the onset of development. These experiments show there is no interconversion between early and late forms in vivo. A one-dimensional peptide map indicated that the early and late forms of alpha-mannosidase have similar amino acid sequences. The two forms have a similar half-life in vivo when measured during the same period of development. Double-labelling studies were performed with 35SO4 and [3H]leucine or 32PO4 and [3H]leucine. and these studies indicated that the oligosaccharides present on the early form of alpha-mannosidase contained more sulphate and phosphate than did those on the late form. The early enzyme had a 10-fold higher 35S/3H ratio and a 4-fold higher 32P/3H ratio. Endocytosis experiments using early and late alpha-mannoside showed that the early form was efficiently taken up by human fibroblasts, whereas the late form was poorly endocytosed. This suggests that the late form lacks the mannose 6-phosphate residue required for efficient uptake.

SUBMITTER: Moore BR 

PROVIDER: S-EPMC1147920 | biostudies-other | 1987 May

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC1161026 | biostudies-other
| S-EPMC1179219 | biostudies-other
| S-EPMC1179340 | biostudies-other
| S-EPMC1360260 | biostudies-literature
| S-EPMC1179339 | biostudies-other
| S-EPMC1177739 | biostudies-other
| S-EPMC1177668 | biostudies-other
| S-EPMC1168002 | biostudies-other
| S-EPMC1184969 | biostudies-other
| S-EPMC1137591 | biostudies-other