Reagents for reversible coupling of proteins to the active centres of trypsin-like serine proteinases.

ABSTRACT: In order to extend the scope for the application of acyl-enzymes as fibrinolytic agents, p-amidinophenyl ester enzyme substrates were prepared that gave stabilized acyl-enzymes capable of being coupled to other proteins. Coupling was achieved by reaction of protein thiol functions with a 2-pyridyldithio moiety within the acyl group. Acyl-enzymes derived from such substrates were stable enough to permit isolation of reversible conjugates between proteins and the active centres of plasminogen activators. Hydrolytic release of active enzyme from a conjugate of human immunoglobulin G and urokinase could be demonstrated.

SUBMITTER: Kalindjian SB 

PROVIDER: S-EPMC1148556 | biostudies-other | 1987 Dec

REPOSITORIES: biostudies-other