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A monoclonal antibody distinguishes two types of phosphatidylinositol 4-kinase.


ABSTRACT: A monoclonal antibody has been developed against the type II PtdIns 4-kinase from bovine brain. This antibody, 4C5G, causes greater than 90% inhibition of the type II PtdIns 4-kinase from bovine brain, rat brain and human erythrocytes. However, it fails to inhibit type III PtdIns 4-kinase from bovine brain or PtdIns 3-kinase from rat liver. These results suggest that type II and type III PtdIns 4-kinases are distinct gene products, and that 4C5G will be useful in studying the function of the type II PtdIns 4-kinase.

SUBMITTER: Endemann GC 

PROVIDER: S-EPMC1149879 | biostudies-other | 1991 Jan

REPOSITORIES: biostudies-other

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