Unknown

Dataset Information

0

Definition of a Ca2(+)-sensitive interface in the plasma gelsolin-actin complex.


ABSTRACT: Gelsolin is a Ca2(+)-dependent protein which severs actin filaments, caps their fast-growing ends and promotes nucleation. We report here results that delimit one of the interfaces between serum gelsolin and actin monomer. An actin-derived synthetic peptide (amino acids 305-326 of actin) coupled to a hydrophilic resin was tested for its possible interaction with gelsolin. We selected this sequence because it corresponds to a region implicated in the gelsolin-actin complex in a previous work [Boyer, Feinberg, Hue, Capony, Benyamin & Roustan (1987) Biochem. J. 248, 359-364]. We showed that this actin sequence is located at the surface of the actin molecule and observed a Ca2(+)-sensitive binding of gelsolin to this actin-derived peptide. In addition, by using a chymotryptic digest of gelsolin, we reported that only the C-terminal half of gelsolin interacts with the actin-(305-326)-peptide. These results that the Ca2(+)-sensitive interface includes both amino acids 305-326 of actin and probably amino acids 660-738 of gelsolin.

SUBMITTER: Houmeida A 

PROVIDER: S-EPMC1149975 | biostudies-other | 1991 Mar

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC1134440 | biostudies-other
| S-EPMC2678046 | biostudies-literature
| S-EPMC4247973 | biostudies-literature
| S-EPMC2905391 | biostudies-literature
| S-EPMC4272649 | biostudies-literature
| S-EPMC6120956 | biostudies-literature
| S-EPMC548556 | biostudies-literature
| S-EPMC1134740 | biostudies-literature
| S-EPMC2719360 | biostudies-literature
| S-EPMC153027 | biostudies-literature