Oxidation of thiol in the vimentin cytoskeleton.
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ABSTRACT: Sublethal doses of H2O2, which induces oxidative stress, cause substantial alteration to the vimentin cytoskeleton in various cell types. We have used a thiol-blot assay to assess thiol status in individual proteins from cell extracts. Vimentin thiol is oxidized in preference to other cytoskeleton proteins. Immunoblot analysis also demonstrated a loss of reactivity to an anti-vimentin monoclonal antibody under non-reducing conditions, possibly due to thiol-group oxidation. During induced oxidative stress a number of proteins become associated with the cytoskeleton extracts.
SUBMITTER: Rogers KR
PROVIDER: S-EPMC1150123 | biostudies-other | 1991 May
REPOSITORIES: biostudies-other
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