Unknown

Dataset Information

0

Oxidation of thiol in the vimentin cytoskeleton.


ABSTRACT: Sublethal doses of H2O2, which induces oxidative stress, cause substantial alteration to the vimentin cytoskeleton in various cell types. We have used a thiol-blot assay to assess thiol status in individual proteins from cell extracts. Vimentin thiol is oxidized in preference to other cytoskeleton proteins. Immunoblot analysis also demonstrated a loss of reactivity to an anti-vimentin monoclonal antibody under non-reducing conditions, possibly due to thiol-group oxidation. During induced oxidative stress a number of proteins become associated with the cytoskeleton extracts.

SUBMITTER: Rogers KR 

PROVIDER: S-EPMC1150123 | biostudies-other | 1991 May

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC11257561 | biostudies-literature
| S-EPMC4708892 | biostudies-literature
| S-EPMC3837443 | biostudies-literature
| S-EPMC3882527 | biostudies-literature
| S-EPMC6413321 | biostudies-literature
| S-EPMC10881836 | biostudies-literature
| S-EPMC3613173 | biostudies-literature
| S-EPMC6755523 | biostudies-literature
| S-EPMC9402923 | biostudies-literature
| S-EPMC2797222 | biostudies-literature