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Synthesis of a gene for rat liver fatty-acid-binding protein and its expression in Escherichia coli.


ABSTRACT: A gene coding for rat liver fatty-acid-binding protein (FABP) has been constructed by the single-step ligation of ten synthetic oligonucleotides. The gene has been cloned into the bacterial expression vector pKK223-3. Induction of protein synthesis from this gene results in over expression of an FABP that is indistinguishable in its structure and binding properties with that isolated from rat liver.

SUBMITTER: Worrall AF 

PROVIDER: S-EPMC1151350 | biostudies-other | 1991 Sep

REPOSITORIES: biostudies-other

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